About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.

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This product complies with the European directives listed below, by fulfilling corresponding standards. A copy of the Declaration of Conformity is available on request. The Warning sign highlights an instruction that must manuak strictly followed in order to avoid personal injury.

Be sure not to proceed until the instructions are clearly understood and all stated conditions are met.

The Caution sign is used to call attention to instructions or conditions that must be followed to avoid damage to the product or other equipment. The Declaration of conformity is valid only for systems that are marked with the CE logo:: The Note sign is used to indicate information important for trouble-free and optimal use of the product.

Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately. Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment. This is a class A product. In a domestic environmentit may cause radio interference, in which case the user may be required to take suitable measures.

All repairs should be done by personnel authorized by by GE Healthcare. The description is common for all the systems, unless otherwise clearly stated in the contents. The differences are few and indicated with divergent typeface. Installation description, system description, system maintenance and troubleshooting are also found in this manual.

The system simplifies the transition from laboratory to full scale production. Scale-up to production is predictable and trouble-free. Brief descriptions of the individual components are given in Section 8. Detailed information on the components can be found in their respective User Manuals and Instructions. The location of the modules and components in the separation units are shown in the following illustrations. The rating label is on the left side and identification labels are on the right viewed from the rear.

This instrument is intended for laboratory use only, not for clinical or in vitro purposes. The system must be connected to a grounded mains socket to prevent system parts from becoming live.

The covers of the modules and components must not be removed by the user. The modules and components contain high voltage circuits that can give a lethal electric shock. Do not disassemble the optical unit while the lamp is ON. Incorrectly fitted tubing may loosen, causing a jet of liquid to spray out. This is especially dangerous if hazardous chemicals are used.


ÄKTApurifier User Guide – GE Healthcare Life Sciences |

Connect the tubing by first inserting the tubing fully, then tightening the connector finger-tight. Do not tighten TeflonTM tubing further as this will damage the end of the tubing. Never place waste containers on top of the system. If they become full and overflow, liquid might penetrate the system causing a shortcircuit. Always disconnect the akat supply before attempting to replace any item on the system during maintenance. In case of an emergency situation, the system mains switch must always be easy to access, and the mains plug must always be easy to disconnect.

When using hazardous chemicals, take all suitable protective measures, such as aktq protective glasses and gloves resistant to the chemicals used.

Follow local regulations and instructions for safe operation and maintenance of the system. Only use spare parts that are approved or supplied by GE Healthcare for maintaining or servicing the system. Only use tubing supplied by GE Healthcare to make sure that the pressure specifications of the tubing are fulfilled.

When using hazardous chemicals, make sure that the entire system has been flushed thoroughly with bacteriostatic solution e. NaOH and distilled water before service and maintenance. For continued protection against risk of fire, replace only with a fuse of the specified type and rating.

Refer to “Reference information” for fuse data. If the system is turned or the fraction collector removed, the external capillaries and other tubing may become entangled in nearby objects and be pulled from their connections causing leakage. This is to prevent liquid spraying out of the ports when switching the valve. When using a sample pump for sample application directly onto the column, a tubing must be connected between ports 3 and 6 on the Injection valve.

Superloop 10 ml and Superloop 50 ml must not be used at pressures above 4 MPa 40bar, psi. Superloop ml must not be used above 2 MPa 20 bar, psi. At higher pressures, the glass tube might shatter.

Never attempt to remove the mains fuse while mains voltage is applied to the system. For continued protection against risk of fire, replace only with fuse of the specified type and rating. Please, refer to the Technical specifications for fuse data. A colon separates menu levels, thus File: Open refers to the Open command in the File menu.

Hardware items, for example, keyboard key names, key combinations, key sequences, hard key names on the equipment and connector port names located on the equipment are identified in the text by bold text. For safe transportation, however, some components have been secured and thus need to be detached before the system can be tested and used. Cables, capillaries, accessories, column holder, etc. It is divided into two parts, one describing the installation and one describing how to run the installation test.

For full details of specifications, methods, maintenance etc. The mains power switch must always be easy to access, and the mains plug must always be easy to disconnect.


Do not block the ventilation inlet on the front panels and the outlets on the rear of the instrument!

ÄKTApurifier User Guide – GE Healthcare Life Sciences

Follow the step-by-step instructions below and check the “Installation Record. Some items are packed in separate boxes delivered with the system. Some packing lists are included in the boxes. Two people are required to lift the system. Do not raise to upright position yet. Lay the system on the same side as on the pallet. Allow purifjer straps to remain taped to the plastic cover.

Also check all enclosed boxes.

Store all the boxes and plastic bags in a convenient nearby place. Pull back the plastic cover 3 to uncover the swivel platform 4 and raise the system to an upright position.


If, for any reason, the equipment has to be repacked, for transportation or otherwise, it is important that the system can be safely packed using the original packing material. Also remove the desiccant bags. Be careful not to cut any capillaries by accident.

Do not lose any of the capillary marking tags. Lift off the valve to remove the protective foam. Refit the valve into the top slot in the attachment bracket. Lift off the mixer to remove the protective foam, and then refit. Lift the optical unit gently to remove the plastic foam packing. Remove all red tape holding capillaries and loops attached to valve INV Remove the red tapes.

Two small lugs on the cover locate in holes at the front and rear of the cell holder. Place the waste tubings marked W1, W2 and W3 in a waste bottle and place the bottle in a convenient place. Unpack the inlet tubing contained in a plastic bag taped to the buffer tray on top of Box Attach the column holder enclosed in Box above the UV cell. Select a slot to suite the height of the column to be used. Select a position that minimizes the distance between the column and the optical unit.

The cell can be placed up to 1. The flow cell itself does not have a recommended flow direction. The dummy pH electrode replaces the pH electrode whenever the pH electrode is to be removed from the system, e.

This is commonly known as a purifidr combination double junction type. The pH electrode is delivered with a transparent cover. The flow cell should not be used with any other pH electrode. Always use the 0. The electrode might rupture due to high backpressure. Secure it with the slide clamp. The optical akra might have to be moved slightly to the left.

The flow direction is marked on the flow cell. Ensure that it is not broken or aktz. Tighten the nut by hand to secure the electrode.